other تاثیر میان کنش مورفین، گلوکز و کانال های پتاسیمی حساس به ATP، بر فرآیند تثبیت مکان شرطی شده ناشی از مورفین تخصصي Special پژوهشي اصیل Research <div style="text-align: justify;"><span style="font-size:12px;"><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;"><strong>هدف: </strong>در تحقیق حاضر تاثیر میان کنش گلوکز</span><span dir="ltr" lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">&nbsp;</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">و کانال های پتاسیمی حساس</span><span dir="ltr" lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">&nbsp;</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">به&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">ATP</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">، بر فرآیند تثبیت ترجیح مکان شرطی شده (</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">CPP</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">) ناشی از مورفین در موش سفید کوچک نر از نژاد&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">NMRI</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">&nbsp;بررسی نشده است.&nbsp;<br> <strong>روش:</strong> برای بررسی اثرات پاداشی مورفین و تاثیر گلوکز، گلیبن کلامید و دیازوکساید بر آن از روش ترجیح مکان شرطی شده استفاده شد.<br> <strong>یافته ها:</strong> تزریق درون صفاقی دوزهای مختلف سولفات مورفین (</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">۰/۵-۷/۵</span><span style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">)،&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">CPP</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">&nbsp;وابسته به دوز ایجاد کرد، در حالی که دوزهای مختلف گلیبن کلامید (۳، ۶ و ۱۲&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">)، دیازوکساید (۱۵، ۳۰ و ۶۰&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">) و گلوکز (۱۰۰، ۲۰۰، ۵۰۰ و</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">۲۰۰۰</span><span style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">) به تنهایی</span><span dir="ltr" lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">&nbsp;</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">چنین تاثیری نداشتند. تزریق گلوکز (</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">۲۰۰۰</span><span style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">) و گلیبن کلامید (</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">۶</span><span style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">) ترجیح مکان شرطی شده ناشی از دوز غیر موثر مورفین (</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">۰/۵</span><span style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">) را معنی دار کرد. از طرف دیگر، دیازوکساید (</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">۶۰</span><span style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">) توانست اثرات گلیبن کلامید (۶&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">) را مهار کند، در حالی که تزریق دیازوکساید به تنهایی تاثیری بر ترجیح مکانی ناشی از مورفین (</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">۰/۵</span><span style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">) نداشت. تزریق دوزهای مختلف گلیبن کلامید (۳، ۶ و&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg ۱۲</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">)، دیازوکساید (۱۵، ۳۰ و</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg ۶۰</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">) و مورفین (</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">mg/kg ۰/۵-۷.۵</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">) تاثیری برمیزان گلوکز خون نداشتند.&nbsp;<br> <strong>نتیجه گیری: </strong>احتمالا گلوکز در ترجیح مکانی ناشی از مورفین از طریق کانال های پتاسیمی حساس به&nbsp;</span><span dir="ltr" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); text-align: right;">ATP</span><span lang="FA" style="margin: 0px; padding: 0px; border: 0px; outline: 0px; vertical-align: baseline; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; color: rgb(0, 0, 0); font-style: normal; text-align: right;">&nbsp;دخالت می کند.</span></span></div> <span style="color: rgb(0, 0, 0); text-transform: none; text-indent: 0px; letter-spacing: normal; font-family: Tahoma; font-size: 10pt; font-style: normal; font-weight: 400; word-spacing: 0px; white-space: normal; orphans: 2; widows: 2; background-color: rgb(255, 255, 255); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;"><strong>Objective: </strong>In the present study interaction of glucose and ATP sensitive potassium channels on the consolidation of morphine induced place preference in male mice has been investigated.<br> <strong>Methods:</strong> Conditioned place preference method was used to assess morphine reward and effects of glucose ,glibenclamide and diazoxide on it.<span>&nbsp;<span>&nbsp;</span></span><br> <strong>Results: </strong>Intraperitoneal administration of different doses of morphine sulphate (0.5-7.5 mg/kg IP) produced a dose-dependent conditioned place preference (cpp).<span>&nbsp;</span></span><span style="color: rgb(0, 0, 0); text-transform: none; text-indent: 0px; letter-spacing: normal; font-family: Tahoma; font-size: 10pt; font-style: normal; font-weight: 400; word-spacing: 0px; white-space: normal; orphans: 2; widows: 2; background-color: rgb(255, 255, 255); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">However, the isolated administration of different doses of glibenclamide (3,6 AND12mg/kg IP), diazoxide (15, 30 and 60mg/kg IP) and glucose (100, 200, 500, 2000 mg/kg) did not produce such an effect. The injection of glucose (2000 mg/kg IP) and glibenclamide (6 mg/kg IP) produced significant morphine (0.5 mg/kg IP) induced cpp. Also, the response of glibenclamide (6mg/kg IP) was attenuated by diazoxide (60 mg /kg IP). However, this dose of diazoxide alone had no effect on morphine (0.5 mg/kg IP) induced cpp .Injection of different doses of diazoxide (15, 30 and 60mg/kg IP), glibenclamide (3, 6 and 12mg/kg IP) and morphine (0.5-7.5 mg/kg) did not effect blood glucose level.<span>&nbsp;</span><br> <strong>Conclusion:</strong></span><span style="color: rgb(0, 0, 0); text-transform: none; text-indent: 0px; letter-spacing: normal; font-family: Tahoma; font-size: 10pt; font-style: normal; font-weight: 400; word-spacing: 0px; white-space: normal; orphans: 2; widows: 2; background-color: rgb(255, 255, 255); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;"><span>&nbsp;</span>It is concluded that glucose may be involved in morphine induced conditioned place preference through KATP channels.</span> مورفین, کانال پتاسیم, تثبیت مکان شرطی شده Morphine, Potassium Channel, Conditional Place Preference 9 16 http://icssjournal.ir/browse.php?a_code=A-10-2-303&slc_lang=other&sid=1 Manoochehr Satari Naeini منوچهر ستاری نایینی 10031947532846003580 10031947532846003580 Yes Department of Science and Research, Islamic Azad University, Tehran, Iran. واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران، ایران. Shahrbanou Oryan شهربانو عریان 10031947532846003581 10031947532846003581 No گروه زیست شناسی دانشگاه تربیت معلم، تهران، ایران. Vahab Babapour وهاب باباپور 10031947532846003582 10031947532846003582 No دانشکده دامپزشکی دانشگاه تهران، تهران، ایران. Mohsen Alibalaie محسن علی بابایی 10031947532846003583 10031947532846003583 No دانشگاه آزاد اسلامی نایین، نایین، ایران.