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Volume 7, Issue 2 (Summer 2005)
Advances in Cognitive Sciences 2005, 7(2): 12-21 |
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Zat Ali H, Zarindast M, Rezayof A, Haeri rouhani A, Razavi movahed S. Interaction of Muscarinic Receptors of Dorsal Hippocampus on Learning Conditioned Place Preference Induced by Morphine in Wistar Rats. Advances in Cognitive Sciences 2005; 7 (2) :12-21
URL: http://icssjournal.ir/article-1-130-en.html
URL: http://icssjournal.ir/article-1-130-en.html
Hengameh Zat Ali 
, Mohammadreza Zarindast * 1, Amaneh Rezayof , Ali Haeri rouhani , Samira Razavi movahed
, Mohammadreza Zarindast * 1, Amaneh Rezayof , Ali Haeri rouhani , Samira Razavi movahed
Abstract: (2181 Views)
Objective: Hippocampus is one of the principal reward – dependent learning centers. Regarding the high density of muscarinic receptors in the CA1 area of dorsal hippocampus, these receptors are likely to be involved in reward-dependent learning. In the present study, the effects of stimulation and / or inhibition of muscarinic receptors in dorsal hippocampus on morphine reward was investigated in male Wistar rats, using the method of Conditioned Place Preference (CPP).
Method: This study was conducted with an experimental method. All animals weighing 200-240 g were bilaterally cannulated in the CA1 area of dorsal hippocampus by a stereotaxic instrument. Each animal was allowed to recover 1-week before CPP processing. A 5-day schedule for CPP was used with three distinct phases: the preconditioning phase, the conditioning phase, which lasted three days, and the testing or expression phase.
Results: The subcutaneous (S.C.) administration of different doses of morphine sulphate (0.5-7 mg/kg) using a 3-day conditioning and unbiased method induced dose-related CPP. Intra-CA1 administration of different doses of physostigmine (anticholinesterase) and atropine (muscarinic antagonist) significantly potentiated and inhibited the morphine induced CPP respectively. Intra-CA1 administration of atropine reversed the potentiation induced by physostigmine in response to morphine.
Conclusion: The single intra-CA1 administration of either physostigmine or atropine alone, did not induce a significant place preference or aversion. It may be concluded that the muscarinic receptors of the CA1 regions of dorsal hippocampus play an important role in morphine reward.
Method: This study was conducted with an experimental method. All animals weighing 200-240 g were bilaterally cannulated in the CA1 area of dorsal hippocampus by a stereotaxic instrument. Each animal was allowed to recover 1-week before CPP processing. A 5-day schedule for CPP was used with three distinct phases: the preconditioning phase, the conditioning phase, which lasted three days, and the testing or expression phase.
Results: The subcutaneous (S.C.) administration of different doses of morphine sulphate (0.5-7 mg/kg) using a 3-day conditioning and unbiased method induced dose-related CPP. Intra-CA1 administration of different doses of physostigmine (anticholinesterase) and atropine (muscarinic antagonist) significantly potentiated and inhibited the morphine induced CPP respectively. Intra-CA1 administration of atropine reversed the potentiation induced by physostigmine in response to morphine.
Conclusion: The single intra-CA1 administration of either physostigmine or atropine alone, did not induce a significant place preference or aversion. It may be concluded that the muscarinic receptors of the CA1 regions of dorsal hippocampus play an important role in morphine reward.
Keywords: Conditioned place preference, Muscarinic receptors, Reward-dependent learning, Dorsal hippocampus
Type of Study: Research |
Subject:
Special
Received: 2005/01/20 | Accepted: 2005/04/21 | Published: 2005/06/22
Received: 2005/01/20 | Accepted: 2005/04/21 | Published: 2005/06/22
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